Talbot (1965) offers the following observations about Stenamma brevicorne from Missouri:
Stenamma brevicorne, together with Lasius flavus, seemed the most typical ant of the low field habitat. It was common and widespread here (23 colonies on 18 plots), but had been collected only once before on the Reserve (at swamp edge). Three other species of Stenamma (S. impar, S. diecki, S. schmitti) present on the Reserve did not live in the field.
Stenamma brevicorne workers foraged over the ground in cool damp weather, and the finding of workers just below the surface indicates that they also foraged below ground in the crumbly topsoil. Nest entrances were simply small openings hidden among leaves and grasses, and were not found unless workers were seen entering them. An attempt was made to collect whole colonies; therefore nests were dug completely when possible. This was not always a successful process, but the general pattern of nest structure seemed clear. Usually there was a chamber within 2 inches of the surface, which might be empty during dry weather. Some nests appeared to have no chambers in the crumbly surface soil but started with a chamber 4 inches down in more solid clay. No colony had more than 4 chambers, and most had only 2. Lowest chambers ranged in depth from 3 to 13 inches, with most at 6 inches. Large colonies dug down to soil moisture, while small colonies did not. Chambers were surprisingly large and were often only partly filled with brood. Those measured ranged from 1/2 X 1/2X 1/4 in. to 1 1/2 X 1 1/2 X 1 in. with most being larger than an inch in diameter and a half inch high. The range of size of the 10 most complete population counts is recorded in Table II. Eggs, larvae and worker pupae were present from before June 23, when the first colonies were dug, through the rest of the summer, although the September 10 colony had only one worker pupa. Twelve colonies which had winged adults or winged pupae averaged 20.6 alates per colony, of which 11.2 were females and 9.4 were males. Winged pupae were found in colonies from July 9 to August 11, and adults from August 10 to September 10 when observations ceased. It is known that S. brevicorne has flights in the spring (Smith, 1957; Kannowski, 1958). It may also fly in the late fall when conditions are right.
This species generally inhabits open areas, with either a soil nest that opens underneath an object resting on the ground or within the litter horizon in debri or other objects where they can aggregate within chamber like structures.